Mechanisms To Assess Gram Stain Interpretation Proficiency of Technologists at Satellite Laboratories

Mechanisms to assess Gram stain interpretation proficiency of technologists at satellite laboratories.

To address Gram stain interpretation proficiency in a satellite/centralized microbiology laboratory paradigm, two programs were devised. In quality assurance program 1, nonmicrobiology technologists at satellite laboratories were required to interpret standardized Gram-stained specimens of clinical material prepared by an experienced microbiologist at a central laboratory. In quality assurance ...

Mechanisms to Assess Gram Stain Proficiency of Technologists at Satellite Laboratories

To address Gram stain proficiency in a satellite/centralized microbiology laboratory paradigm, non-microbiology technologists were required to interpret standardized Gram-stained preparations of clinical material (quality assurance program 1). Clinical Gram stains prepared and read by satellite laboratorians were reviewed by microbiologists (quality assurance program 2). Satisfactory performanc...

Quantitative gram stain interpretation criteria used by microbiology laboratories in Alberta, Canada.

Microbiology laboratories in Alberta, Canada, were surveyed to determine the quantitative interpretive criteria used to routinely read and report Gram stains. There was a wide variability in the quantitative reporting criteria cited for both cells and bacteria, with only 11 of 32 (34.4%) laboratories surveyed using the criteria recommended by the external proficiency-testing program. Lack of st...

The Gram stain.

Mechanism of gram stain reversal.

This paper deals with an investigation of the effect of these reactions on bacteria. Not only the common staining behavior but also certain other similarities (Fischer et al., 1949; Fischer, 1951) between intact wool and gram negative bacteria, medium degraded wool and gram variable bacteria, as well as between highly degraded wool and gram positive bacteria suggested that reversal of gram stai...